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Journal of Biomedical Optics • Open Access

Approach to quantify human dermal skin aging using multiphoton laser scanning microscopy
Author(s): Stefan Puschmann; Christian-Dennis Rahn; Horst Wenck; Stefan Gallinat; Frank F. Fischer

Paper Abstract

Extracellular skin structures in human skin are impaired during intrinsic and extrinsic aging. Assessment of these dermal changes is conducted by subjective clinical evaluation and histological and molecular analysis. We aimed to develop a new parameter for the noninvasive quantitative determination of dermal skin alterations utilizing the high-resolution three-dimensional multiphoton laser scanning microscopy (MPLSM) technique. To quantify structural differences between chronically sun-exposed and sun-protected human skin, the respective collagen-specific second harmonic generation and the elastin-specific autofluorescence signals were recorded in young and elderly volunteers using the MPLSM technique. After image processing, the elastin-to-collagen ratio (ELCOR) was calculated. Results show that the ELCOR parameter of volar forearm skin significantly increases with age. For elderly volunteers, the ELCOR value calculated for the chronically sun-exposed temple area is significantly augmented compared to the sun-protected upper arm area. Based on the MPLSM technology, we introduce the ELCOR parameter as a new means to quantify accurately age-associated alterations in the extracellular matrix.

Paper Details

Date Published: 20 March 2012
PDF: 7 pages
J. Biomed. Opt. 17(3) 036005 doi: 10.1117/1.JBO.17.3.036005
Published in: Journal of Biomedical Optics Volume 17, Issue 3
Show Author Affiliations
Stefan Puschmann, Beiersdorf AG (Germany)
Christian-Dennis Rahn, Beiersdorf AG (Germany)
Horst Wenck, Beiersdorf AG (Germany)
Stefan Gallinat, Beiersdorf AG (Germany)
Frank F. Fischer, Beiersdorf AG (Germany)

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