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Journal of Biomedical Optics • Open Access

Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator

Paper Abstract

Through a combination of a deflective phase-only diffractive spatial light modulator (SLM) and galvo scanners, an addressable multiregional and multifocal multiphoton microscope (AM-MMM) is developed. The SLM shapes an incoming mode-locked, near-infrared Ti:sapphire laser beam into multiple beamlet arrays with addressable shapes and sizes that match the regions of interest on the sample. Compared with conventional multifocal multiphoton microscope (MMM), AM-MMM achieves the effective use of the laser power with an increase of imaging rate and a decrease of photodamage without sacrifice of resolution.

Paper Details

Date Published: 3 April 2012
PDF: 4 pages
J. Biomed. Opt. 17(3) 030505 doi: 10.1117/1.JBO.17.3.030505
Published in: Journal of Biomedical Optics Volume 17, Issue 3
Show Author Affiliations
Yonghong Shao, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)
Xiang Peng, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)
Wan Qin, Clemson Univ. (United States)
Honghai Liu, Clemson Univ. (United States)
Bruce Z. Gao, Clemson Univ. (United States)

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