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Journal of Biomedical Optics

Use of near-infrared luminescent gold nanoclusters for detection of macrophages
Author(s): Veronika Sapozhnikova; Brian Willsey; Li Leo Ma; Keith P. Johnston; Reto Asmis; Tianyi Wang; James T. Jenkins; Jacob Mancuso; Roman V. Kuranov; Thomas Milner; Marc D. Feldman
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Paper Abstract

We determined the effect of aggregation and coating thickness of gold on the luminescence of nanoparticles engulfed by macrophages and in gelatin phantoms. Thin gold-coated iron oxide nanoclusters (nanoroses) have been developed to target macrophages to provide contrast enhancement for near-infrared optical imaging applications. We compare the brightness of nanoroses luminescent emissions in response to 635 nm laser excitation to other nanoparticles including nanoshells, nanorods, and Cy5 conjugated iron oxide nanoparticles. Luminescent properties of all these nanoparticles were investigated in monomeric and aggregated form in gelatin phantoms and primary macrophage cell cultures using confocal microscopy. Aggregation of the gold nanoparticles increased luminescence emission and correlated with increased surface mass of gold per nanoparticle (nanoshells 37±14.30×10−3 brightness with 1.23×10−4 wt of gold (g)/nanoparticle versus original nanorose 1.45±0.37×10−3 with 2.10×10−16 wt of gold/nanoparticle, p<0.05). Nanoshells showed greater luminescent intensity than original nanoroses or Cy5 conjugated iron oxide nanoparticles when compared as nanoparticles per macrophage (38±10 versus 11±2.8 versus 17±6.5, p<0.05, respectively, ANOVA), but showed relatively poor macrophage uptake (1025±128 versus 7549±236 versus 96,000  nanoparticles/cell, p<0.05, student t-test nanoshells versus nanoroses). Enhancement of gold fluorescent emissions by nanoparticles can be achieved by reducing the thickness of the gold coating, by clustering the gold on the surface of the nanoparticles (nanoshells), and by clustering the gold nanoparticles themselves.

Paper Details

Date Published: 7 March 2012
PDF: 13 pages
J. Biomed. Opt. 17(2) 026006 doi: 10.1117/1.JBO.17.2.026006
Published in: Journal of Biomedical Optics Volume 17, Issue 2
Show Author Affiliations
Veronika Sapozhnikova, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Brian Willsey, The Univ. of Texas at Austin (United States)
Li Leo Ma, The Univ. of Texas at Austin (United States)
Keith P. Johnston, The Univ. of Texas at Austin (United States)
Reto Asmis, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Tianyi Wang, The Univ. of Texas at Austin (United States)
James T. Jenkins, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Jacob Mancuso, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Roman V. Kuranov, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Thomas Milner, The Univ. of Texas at Austin (United States)
Marc D. Feldman, The Univ. of Texas Health Science Ctr. at San Antonio (United States)


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