Share Email Print
cover

Journal of Biomedical Optics

Three-dimensional differential interference contrast microscopy using synthetic aperture imaging
Author(s): Moonseok Kim; Youngwoon Choi; Wonshik Choi; Christopher M. Fang-Yen; Yongjin Sung; Ramachandra Rao Dasari; Michael S. Feld; Kwanhyung Kim
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.

Paper Details

Date Published: 23 February 2012
PDF: 7 pages
J. Biomed. Opt. 17(2) 026003 doi: 10.1117/1.JBO.17.2.026003
Published in: Journal of Biomedical Optics Volume 17, Issue 2
Show Author Affiliations
Moonseok Kim, Korea Univ. (Korea, Republic of)
Youngwoon Choi, Korea Univ. (Korea, Republic of)
Wonshik Choi, Korea Univ. (Korea, Republic of)
Christopher M. Fang-Yen, Univ. of Pennsylvania (United States)
Yongjin Sung, Massachusetts Institute of Technology (United States)
Ramachandra Rao Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)
Kwanhyung Kim, LG Electronics Inc. (Korea, Republic of)


© SPIE. Terms of Use
Back to Top