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Journal of Biomedical Optics • Open Access

In vivo near-infrared dual-axis confocal microendoscopy in the human lower gastrointestinal tract

Paper Abstract

Near-infrared confocal microendoscopy is a promising technique for deep in vivo imaging of tissues and can generate high-resolution cross-sectional images at the micron-scale. We demonstrate the use of a dual-axis confocal (DAC) near-infrared fluorescence microendoscope with a 5.5-mm outer diameter for obtaining clinical images of human colorectal mucosa. High-speed two-dimensional en face scanning was achieved through a microelectromechanical systems (MEMS) scanner while a micromotor was used for adjusting the axial focus. In vivo images of human patients are collected at 5  frames/sec with a field of view of 362×212  μm2 and a maximum imaging depth of 140 μm. During routine endoscopy, indocyanine green (ICG) was topically applied a nonspecific optical contrasting agent to regions of the human colon. The DAC microendoscope was then used to obtain microanatomic images of the mucosa by detecting near-infrared fluorescence from ICG. These results suggest that DAC microendoscopy may have utility for visualizing the anatomical and, perhaps, functional changes associated with colorectal pathology for the early detection of colorectal cancer.

Paper Details

Date Published: 2 March 2012
PDF: 5 pages
J. Biomed. Opt. 17(2) 021102 doi: 10.1117/1.JBO.17.2.021102
Published in: Journal of Biomedical Optics Volume 17, Issue 2
Show Author Affiliations
Wibool Piyawattanametha, Stanford Univ. School of Medicine (United States)
Hyejun Ra, Stanford Univ. School of Medicine (United States)
Kevin E. Loewke, Stanford Univ. School of Medicine (United States)
Michael J. Mandella, Stanford Univ. School of Medicine (United States)
Christopher H. Contag, Stanford Univ. School of Medicine (United States)
Zhen Qiu, Univ. of Michigan (United States)
Thomas D. Wang, Univ. of Michigan (United States)
Shai Friedland, Stanford Univ. School of Medicine (United States)
Jonathan T. C. Liu, Stanford Univ. (United States)
Gordon S. Kino, Stony Brook Univ. (United States)
Olav Solgaard, Stony Brook Univ. (United States)

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