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Journal of Biomedical Optics • Open Access

In vivo validation of quantitative frequency domain fluorescence tomography
Author(s): Yuting Lin; Michael T. Ghijsen; Orhan Nalcioglu; Gultekin Gulsen

Paper Abstract

We have developed a hybrid frequency domain fluorescence tomography and magnetic resonance imaging system (MRI) for small animal imaging. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration and lifetime images using a multi-modality approach. In vivo experiments are undertaken to evaluate the system. We compare the recovered fluorescence parameters with and without MRI structural a priori information. In addition, we compare two optical background heterogeneity correction methods: Born normalization and utilizing diffuse optical tomography (DOT) functional a priori information. The results show that the concentration and lifetime of a 4.2-mm diameter indocyanine green inclusion located 15 mm deep inside a rat can be recovered with less than a 5% error when functional a priori information from DOT and structural a priori information from MRI are utilized.

Paper Details

Date Published: 18 December 2012
PDF: 6 pages
J. Biomed. Opt. 17(12) 126021 doi: 10.1117/1.JBO.17.12.126021
Published in: Journal of Biomedical Optics Volume 17, Issue 12
Show Author Affiliations
Yuting Lin, Univ. of California, Irvine (United States)
Michael T. Ghijsen, Univ. of California, Irvine (United States)
Orhan Nalcioglu, Univ. of California, Irvine (United States)
Gultekin Gulsen, Univ. of California, Irvine (United States)

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