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Journal of Biomedical Optics • Open Access

Photoacoustic microscopy of bilirubin in tissue phantoms
Author(s): Yong Zhou; Chi Zhang; Da-Kang Yao; Lihong V. Wang

Paper Abstract

Determining both bilirubin's concentration and its spatial distribution are important in disease diagnosis. Here, for the first time, we applied quantitative multiwavelength photoacoustic microscopy (PAM) to detect bilirubin concentration and distribution simultaneously. By measuring tissue-mimicking phantoms with different bilirubin concentrations, we showed that the root-mean-square error of prediction has reached 0.52 and 0.83  mg/dL for pure bilirubin and for blood-mixed bilirubin detection (with 100% oxygen saturation), respectively. We further demonstrated the capability of the PAM system to image bilirubin distribution both with and without blood. Finally, by underlaying bilirubin phantoms with mouse skins, we showed that bilirubin can be imaged with consistent accuracy down to >400  μm in depth. Our results show that PAM has potential for noninvasive bilirubin monitoring in vivo, as well as for further clinical applications.

Paper Details

Date Published: 13 December 2012
PDF: 7 pages
J. Biomed. Opt. 17(12) 126019 doi: 10.1117/1.JBO.17.12.126019
Published in: Journal of Biomedical Optics Volume 17, Issue 12
Show Author Affiliations
Yong Zhou, Washington Univ. in St. Louis (United States)
Chi Zhang, Washington Univ. in St. Louis (United States)
Da-Kang Yao, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

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