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Journal of Biomedical Optics • Open Access

Improving limited-projection-angle fluorescence molecular tomography using a co-registered x-ray computed tomography scan
Author(s): Karin Radrich; Angelique Ale; Vladimir Ermolayev; Vasilis Ntziachristos

Paper Abstract

We examine the improvement in imaging performance, such as axial resolution and signal localization, when employing limited-projection-angle fluorescence molecular tomography (FMT) together with x-ray computed tomography (XCT) measurements versus stand-alone FMT. For this purpose, we employed living mice, bearing a spontaneous lung tumor model, and imaged them with FMT and XCT under identical geometrical conditions using fluorescent probes for cancer targeting. The XCT data was employed, herein, as structural prior information to guide the FMT reconstruction. Gold standard images were provided by fluorescence images of mouse cryoslices, providing the ground truth in fluorescence bio-distribution. Upon comparison of FMT images versus images reconstructed using hybrid FMT and XCT data, we demonstrate marked improvements in image accuracy. This work relates to currently disseminated FMT systems, using limited projection scans, and can be employed to enhance their performance.

Paper Details

Date Published: 4 December 2012
PDF: 10 pages
J. Biomed. Opt. 17(12) 126011 doi: 10.1117/1.JBO.17.12.126011
Published in: Journal of Biomedical Optics Volume 17, Issue 12
Show Author Affiliations
Karin Radrich, Helmholtz Zentrum München GmbH (Germany)
Angelique Ale, Technische Univ. München (Germany)
Vladimir Ermolayev, Helmholtz Zentrum München GmbH (Germany)
Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany)

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