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Journal of Biomedical Optics

Detection of brain tumors using fluorescence diffuse optical tomography and nanoparticles as contrast agents
Author(s): Pierre-Yves Fortin; Coralie Genevois; Anne Koenig; Emilie Heinrich; Isabelle Texier-Nogues; Franck Couillaud
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Paper Abstract

Near-infrared fluorescence-enhanced diffuse optical tomography (fDOT) is used to localize tumors in mice using fluorescent nanoparticles as a blood pool contrast agent. The infrared dye DiR is loaded in the lipid core of nontargeted nanoparticles (DiR-lipidots) and injected systemically via the tail vein in mice bearing U87 tumors. Distribution and time-course of DiR-lipidots are followed using in vivo fluorescence reflectance imaging and reveal enhanced fluorescent signal within the subcutaneous tumors up to seven days due to the enhanced permeability and retention effect. Tumor growth into the brain is followed using bioluminescent imaging, and tumor localization is further determined by magnetic resonance imaging. The fDOT provides three-dimensional fluorescent maps that allow for consistent localization for both subcutaneous and brain tumors.

Paper Details

Date Published: 3 December 2012
PDF: 6 pages
J. Biomed. Opt. 17(12) 126004 doi: 10.1117/1.JBO.17.12.126004
Published in: Journal of Biomedical Optics Volume 17, Issue 12
Show Author Affiliations
Pierre-Yves Fortin, Univ. Bordeaux Segalen (France)
Coralie Genevois, Univ. Bordeaux Segalen (France)
Anne Koenig, Commissariat à l'Énergie Atomique (France)
Emilie Heinrich, Commissariat à l'Énergie Atomique (France)
Isabelle Texier-Nogues, Commissariat à l'Énergie Atomique (France)
Franck Couillaud, Univ. Bordeaux Segalen (France)


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