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Journal of Biomedical Optics • Open Access

Continuous sensing of tumor-targeted molecular probes with a vertical cavity surface emitting laser-based biosensor
Author(s): Natesh Parashurama; Thomas D. O'Sullivan; Adam de la Zerda; Pascale El Kallassi; Seongjae Cho; Hongguang Liu; Robert Teed; Hart Levy; Jarrett Rosenberg; Zhen Cheng; Ofer Levi; James S. Harris; Sanjiv S. Gambhir

Paper Abstract

Molecular optical imaging is a widespread technique for interrogating molecular events in living subjects. However, current approaches preclude long-term, continuous measurements in awake, mobile subjects, a strategy crucial in several medical conditions. Consequently, we designed a novel, lightweight miniature biosensor for in vivo continuous optical sensing. The biosensor contains an enclosed vertical-cavity surface-emitting semiconductor laser and an adjacent pair of near-infrared optically filtered detectors. We employed two sensors (dual sensing) to simultaneously interrogate normal and diseased tumor sites. Having established the sensors are precise with phantom and in vivo studies, we performed dual, continuous sensing in tumor (human glioblastoma cells) bearing mice using the targeted molecular probe cRGD-Cy5.5, which targets ∝νβ3 cell surface integrins in both tumor neovasculature and tumor. The sensors capture the dynamic time-activity curve of the targeted molecular probe. The average tumor to background ratio after signal calibration for cRGD-Cy5.5 injection is approximately <b<2.43±0.95</b< at 1 h and <b<3.64±1.38</b< at 2 h (<b<<b<N</b<=5</b< mice), consistent with data obtained with a cooled charge coupled device camera. We conclude that our novel, portable, precise biosensor can be used to evaluate both kinetics and steady state levels of molecular probes in various disease applications.

Paper Details

Date Published: 2 November 2012
PDF: 16 pages
J. Biomed. Opt. 17(11) 117004 doi: 10.1117/1.JBO.17.11.117004
Published in: Journal of Biomedical Optics Volume 17, Issue 11
Show Author Affiliations
Natesh Parashurama, Stanford Univ. School of Medicine (United States)
Thomas D. O'Sullivan, Stanford Univ. (United States)
Adam de la Zerda, Stanford Univ. (United States)
Pascale El Kallassi, Stanford Univ. (United States)
Seongjae Cho, Stanford Univ. (United States)
Hongguang Liu, Stanford Univ. (United States)
Robert Teed, Stanford Univ. (United States)
Hart Levy, Univ. of Toronto (Canada)
Jarrett Rosenberg, Stanford Univ. (United States)
Zhen Cheng, Stanford Univ. (United States)
Ofer Levi, Univ. of Toronto (Canada)
James S. Harris, Stanford Univ. (United States)
Sanjiv S. Gambhir, Stanford Univ. (United States)


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