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Journal of Biomedical Optics

Indocyanine-green-loaded microballoons for biliary imaging in cholecystectomy
Author(s): Kinshuk Mitra; James Melvin; Shufang Chang; Ronald Xu; Kyoungjin Park; Alper Yilmaz; Scott Melvin
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Paper Abstract

We encapsulate indocyanine green (ICG) in poly[(D,L-lactide-co-glycolide)-co-PEG] diblock (PLGA-PEG) microballoons for real-time fluorescence and hyperspectral imaging of biliary anatomy. ICG-loaded microballoons show superior fluorescence characteristics and slower degradation in comparison with pure ICG. The use of ICG-loaded microballoons in biliary imaging is demonstrated in both biliary-simulating phantoms and an ex vivo tissue model. The biliary-simulating phantoms are prepared by embedding ICG-loaded microballoons in agar gel and imaged by a fluorescence imaging module in a Da Vinci surgical robot. The ex vivo model consists of liver, gallbladder, common bile duct, and part of the duodenum freshly dissected from a domestic swine. After ICG-loaded microballoons are injected into the gallbladder, the biliary structure is imaged by both hyperspectral and fluorescence imaging modalities. Advanced spectral analysis and image processing algorithms are developed to classify the tissue types and identify the biliary anatomy. While fluorescence imaging provides dynamic information of movement and flow in the surgical region of interest, data from hyperspectral imaging allow for rapid identification of the bile duct and safe exclusion of any contaminant fluorescence from tissue not part of the biliary anatomy. Our experiments demonstrate the technical feasibility of using ICG-loaded microballoons for biliary imaging in

Paper Details

Date Published: 19 November 2012
PDF: 8 pages
J. Biomed. Opt. 17(11) 116025 doi: 10.1117/1.JBO.17.11.116025
Published in: Journal of Biomedical Optics Volume 17, Issue 11
Show Author Affiliations
Kinshuk Mitra, The Ohio State Univ. (United States)
James Melvin, The Ohio State Univ. (United States)
Shufang Chang, The Ohio State Univ. (United States)
Ronald Xu, The Ohio State Univ. (United States)
Kyoungjin Park, The Ohio State Univ. (United States)
Alper Yilmaz, The Ohio State Univ. (United States)
Scott Melvin, The Ohio State Univ. Wexner Medical Ctr. (United States)


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