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Journal of Biomedical Optics • Open Access

In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument
Author(s): Tyler K. Drake; Michael DeSoto; Jennifer J. Peters; Marcus H. Henderson; Bonnie Thiele; Tammy Sinclair Bishop; Amy P. Murtha; David F. Katz; Adam Wax

Paper Abstract

We used a probe-based dual-modality optical imaging instrument to measure in vivo coating thickness distributions of a gel distributed along the vaginal lumen, in a clinical study. The gel was a surrogate for one delivering an anti-HIV topical microbicide. Imaging data from Fourier-domain multiplexed low-coherence interferometry (mLCI) and fluorimetric measurements were compared to assess the feasibility and accuracy of mLCI in measuring in vivo gel coating thickness distributions. In each study session, 3.5 mL of Replens gel was inserted to the vaginal fornix while the participant was supine. The participant either: 1. remained supine (10 or 60 min); or 2. sat up (1 min), stood up (1 min), sat down (1 min) and returned to the supine position; net elapsed time was 10 or 60 min after which the gel distribution was imaged. Local coating thickness distributions were qualitatively and quantitatively similar. Here mLCI did not accurately measure thicker gel coatings (<b<<0.8 mm</b<), a limitation not seen with fluorimetry. However, mLCI is capable of measuring in vivo microbicide gel distributions with resolution on the order of 10 μm, without the need for exogenous contrast agents, and can accurately capture relevant summary coating measures in good agreement with fluorimetry.

Paper Details

Date Published: 1 November 2012
PDF: 7 pages
J. Biomed. Opt. 17(11) 116014 doi: 10.1117/1.JBO.17.11.116014
Published in: Journal of Biomedical Optics Volume 17, Issue 11
Show Author Affiliations
Tyler K. Drake, Duke Univ. (United States)
Michael DeSoto, Duke Univ. (United States)
Jennifer J. Peters, Duke Univ. (United States)
Marcus H. Henderson, Duke Univ. (United States)
Bonnie Thiele, Duke Univ. (United States)
Tammy Sinclair Bishop, Duke Univ. (United States)
Amy P. Murtha, Duke Univ. (United States)
David F. Katz, Duke Univ. (United States)
Adam Wax, Duke Univ. (United States)

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