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Journal of Biomedical Optics

Evaluation of the role of CD207 on Langerhans cells in a murine model of atopic dermatitis by <italic<in situ</italic< imaging using Cr:forsterite laser-based multimodality nonlinear microscopy
Author(s): Jyh-Hong Lee; Ming-Rung Tsai; Chi-Kuang Sun; Bor-Luen Chiang
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Paper Abstract

Atopic dermatitis (AD) is an allergic inflammatory disease of skin. It remains unclear that CD207 of Langerhans cells (LCs) plays a central role in the development of allergic sensitization. There is little data on LCs within the microenviroment <italic<in vivo</italic<. We used a murine model of epicutaneous (EC) ovalbumin (OVA) sensitization inducing an inflammatory skin resembling AD to explore the role of CD207 in the pathogenesis of AD. Cr:forsterite laser-based multimodality nonlinear microscopy was applied for <italic<in situ</italic< imaging. Peritoneal injections of Alexa Fluor 647-rat anti-mouse CD207 into mice were performed to specifically trace the LCs. Peritoneal injections of OVA-Alexa Fluor 647 conjugate into mice were performed to specifically trace the OVA. We found that combining Alexa Fluor fluorescent probes with multimodality nonlinear microscopy permitted the unequivocal <italic<in situ</italic< imaging of CD207-expressing LCs. The relevant time-course, expressional, and functional studies reveal that CD207 of LCs plays an essential role during the induction of EC sensitization. We establish and validate that Cr:forsterite laser-based multimodality nonlinear microscopy is applicable for the specific detection of labeled mAb-bound LCs and labeled antigen. We suggest that CD207-expressing LCs initiate the allergic response through the CD207 mediated epicutaneous sensitization associated with the development of AD.

Paper Details

Date Published: 1 November 2012
PDF: 12 pages
J. Biomed. Opt. 17(11) 116007 doi: 10.1117/1.JBO.17.11.116007
Published in: Journal of Biomedical Optics Volume 17, Issue 11
Show Author Affiliations
Jyh-Hong Lee, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. College of Medicine (Taiwan)
Ming-Rung Tsai, National Taiwan University (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)
Research Ctr. for Applied Sciences (Taiwan)
Bor-Luen Chiang, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. College of Medicine (Taiwan)


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