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Journal of Biomedical Optics

In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma
Author(s): Yang Sun; Hongtao Xie; Jing Liu; Matthew Lam; Abhijit J. Chaudhari; Feifei Zhou; Julien Bec; Diego R. Yankelevich; Allison Dobbie; Steven P. Tinling; Regina Gandour-Edwards; Wayne L. Monsky; Gregory D. Farwell; Laura Marcu
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Paper Abstract

Tissue diagnostic features generated by a bimodal technique integrating scanning time-resolved fluorescence spectroscopy (TRFS) and ultrasonic backscatter microscopy (UBM) are investigated in an in vivo hamster oral carcinoma model. Tissue fluorescence is excited by a pulsed nitrogen laser and spectrally and temporally resolved using a set of filters/dichroic mirrors and a fast digitizer, respectively. A 41-MHz focused transducer (37-μm axial, 65-μmm lateral resolution) is used for UBM scanning. Representative lesions of the different stages of carcinogenesis show that fluorescence characteristics complement ultrasonic features, and both correlate with histological findings. These results demonstrate that TRFS-UBM provide a wealth of co-registered, complementary data concerning tissue composition and structure as it relates to disease status. The direct co-registration of the TRFS data (sensitive to surface molecular changes) with the UBM data (sensitive to cross-sectional structural changes and depth of tumor invasion) is expected to play an important role in pre-operative diagnosis and intra-operative determination of tumor margins.

Paper Details

Date Published: 1 November 2012
PDF: 11 pages
J. Biomed. Opt. 17(11) 116003 doi: 10.1117/1.JBO.17.11.116003
Published in: Journal of Biomedical Optics Volume 17, Issue 11
Show Author Affiliations
Yang Sun, Univ. of California, Davis (United States)
Hongtao Xie, Univ. of California, Davis (United States)
Jing Liu, Univ. of California, Davis (United States)
Matthew Lam, Univ. of California, Davis (United States)
Abhijit J. Chaudhari, Univ. of California, Davis (United States)
Feifei Zhou, Univ. of California, Davis (United States)
Julien Bec, Univ. of California, Davis (United States)
Diego R. Yankelevich, Univ. of California, Davis (United States)
Allison Dobbie, Univ. of California, Davis (United States)
Steven P. Tinling, Univ. of California, Davis (United States)
Regina Gandour-Edwards, Univ. of California, Davis (United States)
Wayne L. Monsky, Univ. of California, Davis (United States)
Gregory D. Farwell, Univ. of California, Davis (United States)
Laura Marcu, Univ. of California, Davis (United States)


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