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Journal of Biomedical Optics • Open Access

Complementary fluorescence-polarization microscopy using division-of-focal-plane polarization imaging sensor
Author(s): Yang Liu; Timothy York; Walter J. Akers; Gail P. Sudlow; Viktor Gruev; Samuel Achilefu

Paper Abstract

Fluorescence microscopy offers high sensitivity for disease diagnosis. However, little structural information is revealed by this method, requiring another technique to localize the source of fluorescence. We developed a complementary fluorescence-polarization microscope. We used a division-of-focal-plane charge coupled device polarization sensor to enable real-time video rate polarization imaging without any moving parts. The polarization information provided by the microscope enabled detection of structural element and complements the fluorescence information. Application of this multimodal system for cancer imaging using a tumor selective molecular probe revealed the association of diminished structural integrity of tumor tissue with high fluorescence of the imaging agent compared to surrounding normal tissue. This study demonstrates a new paradigm to improve cancer detection and diagnosis.

Paper Details

Date Published: 1 November 2012
PDF: 4 pages
J. Biomed. Opt. 17(11) 116001 doi: 10.1117/1.JBO.17.11.116001
Published in: Journal of Biomedical Optics Volume 17, Issue 11
Show Author Affiliations
Yang Liu, Washington Univ. in St. Louis (United States)
Timothy York, Washington Univ. in St. Louis (United States)
Walter J. Akers, Washington Univ. School of Medicine in St. Louis (United States)
Gail P. Sudlow, Washington Univ. in St. Louis (United States)
Viktor Gruev, Washington Univ. in St. Louis (United States)
Samuel Achilefu, Washington Univ. School of Medicine in St. Louis (United States)


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