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Journal of Biomedical Optics

Real-time, in vivo measurement of tissular pO2 through the delayed fluorescence of endogenous protoporphyrin IX during photodynamic therapy
Author(s): Filippo M. Piffaretti; Anna Maria Novello; Rajendran Senthil Kumar; Eddy Forte; Cédric Paulou; Patrycja M. Nowak-Sliwinska; Hubert E. van den Bergh; Georges A. Wagnières
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Paper Abstract

Tissular oxygen concentration plays a key role during photodynamic therapy (PDT). Therefore, monitoring its local oxygen partial pressure (pO2) may help predict and/or control the outcome of a PDT treatment. The first real-time, in vivo measurements of the pO2 in the chicken egg's chorioallantoic membrane, using the delayed fluorescence of photoactivable porphyrins (PAPs), including protoporphyrin IX (PpIX), as monitored with a dedicated optical, fiber-based, time-resolved spectrometer, are reported here. The formation of PAPs/PpIX, photosensitizers of extensive clinical use, was induced in the chicken egg's chorioallantoic membrane (CAM) with aminolevulinic acid. An excellent correlation between the vascular damage induced by PDT and the reduction in tissular pO2 is found. This study suggests that clinical measurement of the pO2 using the PAPs'/PpIX's delayed fluorescence (DF) may be used to individualize in real time the PDT light dose applied.

Paper Details

Date Published: 9 November 2012
PDF: 9 pages
J. Biomed. Opt. 17(11) 115007 doi: 10.1117/1.JBO.17.11.115007
Published in: Journal of Biomedical Optics Volume 17, Issue 11
Show Author Affiliations
Filippo M. Piffaretti, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Anna Maria Novello, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Rajendran Senthil Kumar, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Eddy Forte, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Cédric Paulou, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Patrycja M. Nowak-Sliwinska, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Hubert E. van den Bergh, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Georges A. Wagnières, Ecole Polytechnique Fédérale de Lausanne (Switzerland)


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