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Journal of Biomedical Optics

Reagent-free bacterial identification using multivariate analysis of transmission spectra
Author(s): Jennifer M. Smith; Debra E. Huffman; Dayanis Acosta; Yulia Serebrennikova; Luis H. Garcia-Rubio; German F. Leparc
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Paper Abstract

The identification of bacterial pathogens from culture is critical to the proper administration of antibiotics and patient treatment. Many of the tests currently used in the clinical microbiology laboratory for bacterial identification today can be highly sensitive and specific; however, they have the additional burdens of complexity, cost, and the need for specialized reagents. We present an innovative, reagent-free method for the identification of pathogens from culture. A clinical study has been initiated to evaluate the sensitivity and specificity of this approach. Multiwavelength transmission spectra were generated from a set of clinical isolates including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Spectra of an initial training set of these target organisms were used to create identification models representing the spectral variability of each species using multivariate statistical techniques. Next, the spectra of the blinded isolates of targeted species were identified using the model achieving >94% sensitivity and >98% specificity, with 100% accuracy for P. aeruginosa and S. aureus. The results from this on-going clinical study indicate this approach is a powerful and exciting technique for identification of pathogens. The menu of models is being expanded to include other bacterial genera and species of clinical significance.

Paper Details

Date Published: 1 October 2012
PDF: 9 pages
J. Biomed. Opt. 17(10) 107002 doi: 10.1117/1.JBO.17.10.107002
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Jennifer M. Smith, Claro Scientific, LLC (United States)
Debra E. Huffman, Claro Scientific, LLC (United States)
Dayanis Acosta, Claro Scientific, LLC (United States)
Yulia Serebrennikova, Claro Scientific, LLC (United States)
Luis H. Garcia-Rubio, Claro Scientific, LLC (United States)
German F. Leparc, Florida Blood Services (United States)

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