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Journal of Biomedical Optics • Open Access

Real-time study of shape and thermal fluctuations in the echinocyte transformation of human erythrocytes using defocusing microscopy
Author(s): Sebastian Etcheverry; Maria Jose Gallardo; Pablo Solano; Mario Suwalsky; Oscar N. Mesquita; Carlos Saavedra

Paper Abstract

We present a real-time method to measure the amplitude of thermal fluctuations in biological membranes by means of a new treatment of the defocusing microscopy (DM) optical technique. This approach was also applied to study the deformation of human erythrocytes to its echinocyte structure. This was carried out by making three-dimensional shape reconstructions of the cell and measuring the thermal fluctuations of its membrane, as the cell is exposed to the anti-inflammatory drug naproxen and as it recovers its original shape, when it is subsequently cleansed of the drug. The results showed biomechanical changes in the membrane even at low naproxen concentration (0.2 mM). Also, we found that when the cell recovered its original shape, the membrane properties were different compared to the nondrugged initial erythrocyte, indicating that the drug administration-recovery process is not completely reversible.

Paper Details

Date Published: 4 October 2012
PDF: 6 pages
J. Biomed. Opt. 17(10) 106013 doi: 10.1117/1.JBO.17.10.106013
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Sebastian Etcheverry, Univ. de Concepción (Chile)
Maria Jose Gallardo, Univ. de Concepción (Chile)
Pablo Solano, Univ. de Concepción (Chile)
Mario Suwalsky, Univ. de Concepción (Chile)
Oscar N. Mesquita, Univ. Federal de Minas Gerais (Brazil)
Carlos Saavedra, Univ. de Concepción (Chile)

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