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Journal of Biomedical Optics • Open Access

Evaluation and quantification of spectral information in tissue by confocal microscopy
Author(s): Ulf Maeder; Kay Marquardt; Sebastian Beer; Thorsten Bergmann; Thomas Schmidts; Johannes T. Heverhagen; Klemens Zink; Frank Runkel; Martin Fiebich

Paper Abstract

A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The processing scheme is based on spectral data, is less error-prone than intensity-based visualization and evaluation methods, and provides quantitative information on the composition of the sample. The method is tested and validated in the context of the development of dermal drug delivery systems, introducing a quantitative uptake indicator to compare the performances of different delivery systems is introduced. A drug penetration study was performed in vitro. The results show that the method is able to detect, visualize and measure spectral information in tissue. In the penetration study, uptake efficiencies of different experiment setups could be discriminated and quantitatively described. The developed uptake indicator is a step towards a quantitative assessment and, in a more general view apart from pharmaceutical research, provides valuable information on tissue composition. It can potentially be used for clinical in vitro and in vivo applications.

Paper Details

Date Published: 1 October 2012
PDF: 8 pages
J. Biomed. Opt. 17(10) 106011 doi: 10.1117/1.JBO.17.10.106011
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Ulf Maeder, Technische Hochschule Mittelhessen (Germany)
Kay Marquardt, Technische Hochschule Mittelhessen (Germany)
Sebastian Beer, Technische Hochschule Mittelhessen (Germany)
Thorsten Bergmann, Technische Hochschule Mittelhessen (Germany)
Thomas Schmidts, Technische Hochschule Mittelhessen (Germany)
Johannes T. Heverhagen, Univ. Hospital Inselspital (Switzerland)
Klemens Zink, Technische Hochschule Mittelhessen (Germany)
Frank Runkel, Technische Hochschule Mittelhessen (Germany)
Martin Fiebich, Technische Hochschule Mittelhessen (Germany)

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