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Journal of Biomedical Optics • Open Access

Near-infrared fluorescence imaging of experimentally collagen-induced arthritis in rats using the nonspecific dye tetrasulfocyanine in comparison with gadolinium-based contrast-enhanced magnetic resonance imaging, histology, and clinical score
Author(s): Ines Gemeinhardt; Dorothee Puls; Ole Gemeinhardt; Matthias Taupitz; Susanne Wagner; Beatrix Schnorr; Kai Licha; Michael Schirner; Bernd Ebert; Diethard Petzelt; Rainer Macdonald; Jörg Schnorr

Paper Abstract

Using 15 rats with collagen-induced arthritis (30 joints) and 7 control rats (14 joints), we correlated the intensity of near-infrared fluorescence (NIRF) of the nonspecific dye tetrasulfocyanine (TSC) with magnetic resonance imaging (MRI), histopathology, and clinical score. Fluorescence images were obtained in reflection geometry using a NIRF camera system. Normalized fluorescence intensity (I NF ) was determined after intravenous dye administration on different time points up to 120 min. Contrast-enhanced MRI using gadodiamide was performed after NIRF imaging. Analyses were performed in a blinded fashion. Histopathological and clinical scores were determined for each ankle joint. I NF of moderate and high-grade arthritic joints were significantly higher (p<0.005 ) than the values of control and low-grade arthritic joints between 5 and 30 min after TSC-injection. This result correlated well with post-contrast MRI signal intensities at about 5 min after gadodiamide administration. Furthermore, I NF and signal increase on contrast-enhanced MRI showed high correlation with clinical and histopathological scores. Sensitivities and specificities for detection of moderate and high-grade arthritic joints were slightly lower for NIRF imaging (89%/81% ) than for MRI (100%/91% ). NIRF imaging using TSC, which is characterized by slower plasma clearance compared to indocyanine green (ICG), has the potential to improve monitoring of inflamed joints.

Paper Details

Date Published: 1 October 2012
PDF: 9 pages
J. Biomed. Opt. 17(10) 106008 doi: 10.1117/1.JBO.17.10.106008
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Ines Gemeinhardt, Charité Universitätsmedizin Berlin (Germany)
Dorothee Puls, Charité Universitätsmedizin Berlin (Germany)
Ole Gemeinhardt, Charité Universitätsmedizin Berlin (Germany)
Matthias Taupitz, Charité Universitätsmedizin Berlin (Germany)
Susanne Wagner, Charité Universitätsmedizin Berlin (Germany)
Beatrix Schnorr, Charité Universitätsmedizin Berlin (Germany)
Kai Licha, mivenion GmbH (Germany)
Michael Schirner, mivenion GmbH (Germany)
Bernd Ebert, Physikalisch-Technische Bundesanstalt (Germany)
Diethard Petzelt, Physikalisch-Technische Bundesanstalt (Germany)
Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany)
Jörg Schnorr, Charité Universitätsmedizin Berlin (Germany)

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