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Journal of Biomedical Optics

Video-rate functional photoacoustic microscopy at depths
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Paper Abstract

We report the development of functional photoacoustic microscopy capable of video-rate high-resolution in vivo imaging in deep tissue. A lightweight photoacoustic probe is made of a single-element broadband ultrasound transducer, a compact photoacoustic beam combiner, and a bright-field light delivery system. Focused broadband ultrasound detection provides a 44-μm lateral resolution and a 28-μm axial resolution based on the envelope (a 15-μm axial resolution based on the raw RF signal). Due to the efficient bright-field light delivery, the system can image as deep as 4.8 mm in vivo using low excitation pulse energy (28 μJ per pulse, 0.35  mJ/cm 2 on the skin surface). The photoacoustic probe is mounted on a fast-scanning voice-coil scanner to acquire 40 two-dimensional (2-D) B-scan images per second over a 9-mm range. High-resolution anatomical imaging is demonstrated in the mouse ear and brain. Via fast dual-wavelength switching, oxygen dynamics of mouse cardio-vasculature is imaged in realtime as well.

Paper Details

Date Published: 1 October 2012
PDF: 5 pages
J. Biomed. Opt. 17(10) 106007 doi: 10.1117/1.JBO.17.10.106007
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Lidai Wang, Washington Univ. School of Medicine in St. Louis (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Wenxin Xing, Washington Univ. in St. Louis (United States)
Alejandro Garcia-Uribe, Washington Univ. School of Medicine in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


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