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Journal of Biomedical Optics • Open Access

Optical molecular imaging approach for rapid assessment of response of individual cancer cells to chemotherapy
Author(s): Zhen Luo; Rohan Vijay Tikekar; Kiana Michelle Samadzadeh; Nitin Nitin

Paper Abstract

Predicting the response of individual patients to cytotoxic chemotherapy drugs is critical for developing individualized therapies. With this motivation, an optical molecular imaging approach was developed to detect cisplatin induced changes in the uptake and intracellular retention of choline. Intracellular uptake of choline was characterized using a click chemistry reaction between propargyl choline and Alexa-488 azide. Cisplatin induced changes in the uptake of propargyl choline in cells and tumor spheroids were compared with similar measurements using a fluorescent analogue of deoxyglucose and conventional cell viability assays. Uptake and intracellular retention of propargyl choline decreased with an increase in concentration of cisplatin. Intracellular uptake of propargyl choline was significantly reduced within 3 h of incubation with a sub-lethal dose of cisplatin. Results demonstrate that the imaging approach based on propargyl choline was more sensitive in detecting the early response of cancer cells to cisplatin as compared to the imaging based on fluorescent analogue of deoxyglucose and cell viability assays. Imaging measurements in tumor spheroids show a significant decrease in the uptake of propargyl choline following treatment with cisplatin. Overall, the results demonstrate a novel optical molecular imaging approach for rapid measurement of the response of individual cancer cells to cisplatin treatment.

Paper Details

Date Published: 1 October 2012
PDF: 8 pages
J. Biomed. Opt. 17(10) 106006 doi: 10.1117/1.JBO.17.10.106006
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Zhen Luo, Univ. of California, Davis (United States)
Rohan Vijay Tikekar, Univ. of California, Davis (United States)
Kiana Michelle Samadzadeh, Univ. of California, Davis (United States)
Nitin Nitin, Univ. of California, Davis (United States)


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