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Journal of Biomedical Optics

In vivo Raman spectroscopic identification of premalignant lesions in oral buccal mucosa
Author(s): S. P. Singh; Atul Deshmukh; Pankaj Chaturvedi; C. Murali Krishna
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Paper Abstract

Cancers of oral cavities are one of the most common malignancies in India and other south-Asian countries. Tobacco habits are the main etiological factors for oral cancer. Identification of premalignant lesions is required for improving survival rates related to oral cancer. Optical spectroscopy methods are projected as alternative/adjunct for cancer diagnosis. Earlier studies have demonstrated the feasibility of classifying normal, premalignant, and malignant oral ex-vivo tissues. We intend to evaluate potentials of Raman spectroscopy in detecting premalignant conditions. Spectra were recorded from premalignant patches, contralateral normal (opposite to tumor site), and cancerous sites of subjects with oral cancers and also from age-matched healthy subjects with and without tobacco habits. A total of 861 spectra from 104 subjects were recorded using a fiber-optic probe-coupled HE-785 Raman spectrometer. Spectral differences in the 1200- to 1800-cm −1 region were subjected to unsupervised principal component analysis and supervised linear discriminant analysis followed by validation with leave-one-out and an independent test data set. Results suggest that premalignant conditions can be objectively discriminated with both normal and cancerous sites as well as from healthy controls with and without tobacco habits. Findings of the study further support efficacy of Raman spectroscopic approaches in oral-cancer applications.

Paper Details

Date Published: 1 October 2012
PDF: 9 pages
J. Biomed. Opt. 17(10) 105002 doi: 10.1117/1.JBO.17.10.105002
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
S. P. Singh, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Atul Deshmukh, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Pankaj Chaturvedi, Tata Memorial Hospital (India)
C. Murali Krishna, Advanced Ctr. for Treatment, Research & Education in Cancer (India)


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