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Journal of Biomedical Optics • Open Access

Trapping and dynamic manipulation of polystyrene beads mimicking circulating tumor cells using targeted magnetic/photoacoustic contrast agents
Author(s): Chen-Wei Wei; Jinjun Xia; Ivan M. Pelivanov; Xiaoge Hu; Xiaohu Gao; Matthew O'Donnell

Paper Abstract

Results on magnetically trapping and manipulating micro-scale beads circulating in a flow field mimicking metastatic cancer cells in human peripheral vessels are presented. Composite contrast agents combining magneto-sensitive nanospheres and highly optical absorptive gold nanorods were conjugated to micro-scale polystyrene beads. To efficiently trap the targeted objects in a fast stream, a dual magnet system consisting of two flat magnets to magnetize (polarize) the contrast agent and an array of cone magnets producing a sharp gradient field to trap the magnetized contrast agent was designed and constructed. A water-ink solution with an optical absorption coefficient of 10  cm−1 was used to mimic the optical absorption of blood. Magnetomotive photoacoustic imaging helped visualize bead trapping, dynamic manipulation of trapped beads in a flow field, and the subtraction of stationary background signals insensitive to the magnetic field. The results show that trafficking micro-scale objects can be effectively trapped in a stream with a flow rate up to 12  ml/min and the background can be significantly (greater than 15 dB) suppressed. It makes the proposed method very promising for sensitive detection of rare circulating tumor cells within high flow vessels with a highly absorptive optical background.

Paper Details

Date Published: 14 August 2012
PDF: 7 pages
J. Biomed. Opt. 17(10) 101517 doi: 10.1117/1.JBO.17.10.101517
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Chen-Wei Wei, Univ. of Washington (United States)
Jinjun Xia, Univ. of Washington (United States)
Ivan M. Pelivanov, Univ. of Washington (Uganda)
Xiaoge Hu, Univ. of Washington (United States)
Xiaohu Gao, Univ. of Washington (United States)
Matthew O'Donnell, Univ. of Washington (United States)

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