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Journal of Biomedical Optics • Open Access

Multi-parametric imaging of murine brain using spectral and time domain optical coherence tomography

Paper Abstract

Examination of brain functions in small animal models may help improve the diagnosis and treatment of neurological conditions. Transcranial imaging of small rodents' brains poses a major challenge for optical microscopy. Another challenge is to reduce the measurement time. We describe methods and algorithms for three-dimensional assessment of blood flow in the brains of small animals, through the intact skull, using spectral and time domain optical coherence tomography. By introducing a resonant scanner to the optical setup of the optical coherence tomography (OCT) system, we have developed and applied a high-speed spectral OCT technique that allows us to vary the imaging range of flow and to shorten measurement time. Multi-parameter signal analysis enables us to obtain both qualitative and quantitative information about flow velocity from the same set of data.

Paper Details

Date Published: 14 June 2012
PDF: 5 pages
J. Biomed. Opt. 17(10) 101515 doi: 10.1117/1.JBO.17.10.101515
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Danuta Bukowska, Nicolaus Copernicus Univ. (Poland)
Daniel Ruminski, Nicolaus Copernicus Univ. (Poland)
Daniel Szlag, Nicolaus Copernicus Univ. (Poland)
Ireneusz Grulkowski, Nicolaus Copernicus Univ. (Poland)
Maciej Szkulmowski, Nicolaus Copernicus Univ. (Poland)
Iwona M. Gorczynska, Nicolaus Copernicus Univ. (Poland)
Maciej Wojtkowski, Nicolaus Copernicus Univ. (Poland)
Jakub Wlodarczyk, Nencki Institute of Experimental Biology (Poland)
Grzegorz Wilczynski, Nencki Institute of Experimental Biology (Poland)

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