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Journal of Biomedical Optics • Open Access

Optical phase nanoscopy in red blood cells using low-coherence spectroscopy
Author(s): Itay Shock; Pinhas Girshovitz; Uri Nevo; Natan T. Shaked; Alexander Barbul; Rafi Korenstein

Paper Abstract

We propose a low-coherence spectral-domain phase microscopy (SDPM) system for accurate quantitative phase measurements in red blood cells (RBCs) for the prognosis and monitoring of disease conditions that affect the visco-elastic properties of RBCs. Using the system, we performed time-recordings of cell membrane fluctuations, and compared the nano-scale fluctuation dynamics of healthy and glutaraldehyde-treated RBCs. Glutaraldehyde-treated RBCs possess lower amplitudes of fluctuations, reflecting an increased membrane stiffness. To demonstrate the ability of our system to measure fluctuations of lower amplitudes than those measured by the commonly used holographic phase microscopy techniques, we also constructed wide-field digital interferometry (WFDI) system and compared the performances of both systems. Due to its common-path geometry, the optical-path-delay stability of SDPM was found to be less than 0.3 nm in liquid environment, at least three times better than WFDI under the same conditions. In addition, due to the compactness of SDPM and its inexpensive and robust design, the system possesses a high potential for clinical applications.

Paper Details

Date Published: 13 June 2012
PDF: 6 pages
J. Biomed. Opt. 17(10) 101509 doi: 10.1117/1.JBO.17.10.101509
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Itay Shock, Tel Aviv Univ. (Israel)
Pinhas Girshovitz, Tel Aviv Univ. (Israel)
Uri Nevo, Tel Aviv Univ. (Israel)
Natan T. Shaked, Tel Aviv Univ. (Israel)
Alexander Barbul, Tel Aviv Univ. (Israel)
Rafi Korenstein, Tel Aviv Univ. (Israel)


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