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Journal of Biomedical Optics • Open Access

Tumor cell differentiation by label-free fluorescence microscopy
Author(s): Petra Weber; Michael Wagner; Herbert Schneckenburger; Petra Kioschis; Waltraud Kessler

Paper Abstract

Autofluorescence spectra, images, and decay kinetics of U251-MG glioblastoma cells prior and subsequent to activation of tumor suppressor genes are compared. While phase contrast images and fluorescence intensity patterns of tumor (control) cells and less malignant cells are similar, differences can be deduced from autofluorescence spectra and decay kinetics. In particular, upon near UV excitation, the fluorescence ratio of the free and protein-bound coenzyme nicotinamid adenine dinucleotide depends on the state of malignancy and reflects different cytoplasmic (including lysosomal) and mitochondrial contributions. While larger numbers of fluorescence spectra are evaluated by principal component analysis, a multivariate data analysis method, additional information on cell metabolism is obtained from spectral imaging and fluorescence lifetime imaging microscopy.

Paper Details

Date Published: 11 June 2012
PDF: 6 pages
J. Biomed. Opt. 17(10) 101508 doi: 10.1117/1.JBO.17.10.101508
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Petra Weber, Hochschule Aalen (Germany)
Michael Wagner, Hochschule Aalen (Germany)
Herbert Schneckenburger, Hochschule Aalen (Germany)
Petra Kioschis, Hochschule Mannheim (Germany)
Waltraud Kessler, Steinbeis-Transfer-Institut Multivariate Datenanalyse (Germany)

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