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Journal of Biomedical Optics • Open Access

Metabolism of HeLa cells revealed through autofluorescence lifetime upon infection with enterohemorrhagic Escherichia coli
Author(s): Tatyana Buryakina; Pin-Tzu Su; Wan-Jr Syu; C. Allen Chang; Hsui-Fang Fan; Fu-Jen Kao

Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a sensitive technique in monitoring functional and conformational states of nicotinamide adenine dinucleotide reduced (NADH) and flavin adenine dinucleotide (FAD),main compounds participating in oxidative phosphorylation in cells. In this study, we have applied FLIM to characterize the metabolic changes in HeLa cells upon bacterial infection and made comparison with the results from the cells treated with staurosporine (STS), a well-known apoptosis inducer. The evolving of NADH's average autofluorescence lifetime during the 3 h after infection with enterohemorragic Escherichia coli (EHEC) or STS treatment has been observed. The ratio of the short and the long lifetime components' relative contributions of NADH increases with time, a fact indicating cellular metabolic activity, such as a decrease of oxidative phosphorylation over the course of infection, while opposite dynamics is observed in FAD. Being associated with mitochondria, FAD lifetimes and redox ratio could indicate heterogeneous mitochondrial function, microenvironment with bacterial infection, and further pathway to cell death. The redox ratios for both EHEC-infected and STS-treated HeLa cells have been observed and these observations also indicate possible apoptosis induced by bacterial infection.

Paper Details

Date Published: 21 May 2012
PDF: 8 pages
J. Biomed. Opt. 17(10) 101503 doi: 10.1117/1.JBO.17.10.101503
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Tatyana Buryakina, National Yang-Ming Univ. (Taiwan)
Pin-Tzu Su, National Yang-Ming Univ. (Taiwan)
Wan-Jr Syu, National Yang-Ming Univ. (Taiwan)
C. Allen Chang, National Yang-Ming Univ. (Taiwan)
Hsui-Fang Fan, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

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