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Journal of Biomedical Optics

Nanosurgery of cells and chromosomes using near-infrared twelve-femtosecond laser pulses
Author(s): Aisada Uchugonova; Karsten König; Matthias Lessel; Christian Zeitz; Karin Jacobs; Sander Nietzsche; Cornelius Lemke
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Paper Abstract

Laser-assisted surgery based on multiphoton absorption of near-infrared laser light has great potential for high precision surgery at various depths within the cells and tissues. Clinical applications include refractive surgery (fs-LASIK). The non-contact laser method also supports contamination-free cell nanosurgery. In this paper we describe usage of an ultrashort femtosecond laser scanning microscope for sub-100 nm surgery of human cells and metaphase chromosomes. A mode-locked 85 MHz Ti:Sapphire laser with an M-shaped ultrabroad band spectrum (maxima: 770  nm/830  nm) and an in situ pulse duration at the target ranging from 12 fs up to 3 ps was employed. The effects of laser nanoprocessing in cells and chromosomes have been quantified by atomic force microscopy. These studies demonstrate the potential of extreme ultrashort femtosecond laser pulses at low mean milliwatt powers for sub-100 nm surgery of cells and cellular organelles.

Paper Details

Date Published: 16 May 2012
PDF: 7 pages
J. Biomed. Opt. 17(10) 101502 doi: 10.1117/1.JBO.17.10.101502
Published in: Journal of Biomedical Optics Volume 17, Issue 10
Show Author Affiliations
Aisada Uchugonova, Univ. des Saarlandes (Germany)
Karsten König, Univ. des Saarlandes (Germany)
Matthias Lessel, Univ. des Saarlandes (Germany)
Christian Zeitz, Univ. des Saarlandes (Germany)
Karin Jacobs, Univ. des Saarlandes (Germany)
Sander Nietzsche, Universitätsklinikum Jena (Germany)
Cornelius Lemke, Friedrich-Schiller-Univ. Jena (Germany)

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