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Journal of Biomedical Optics • Open Access

Establishment of rules for interpreting ultraviolet autofluorescence microscopy images for noninvasive detection of Barrett's esophagus and dysplasia
Author(s): Bevin Lin; Dennis L. Matthews; Stavros G. Demos; Shiro Urayama; Ramez M. G. Saroufeem

Paper Abstract

The diagnostic potential of autofluorescence (AF) microscopy under ultraviolet (UV) excitation is explored using ex vivo human specimens. The aim is to establish optical patterns (the rules for interpretation) that correspond to normal and abnormal histologies of the esophagus, spanning from early benign modifications (Barrett's esophagus) to subsequent dysplastic change and progression toward carcinoma. This was achieved by developing an image library categorized by disease progression. We considered morphological changes of disease as they are compared with histological diagnosis of the pathological specimen, as well as control samples of normal esophagus, proximal stomach, and small intestine tissue. Our experimental results indicate that UV AF microscopy could provide real-time histological information for visualizing changes in tissue microstructure that are currently undetectable using conventional endoscopic methods.

Paper Details

Date Published: 13 February 2012
PDF: 11 pages
J. Biomed. Opt. 17(1) 016013 doi: 10.1117/1.JBO.17.1.016013
Published in: Journal of Biomedical Optics Volume 17, Issue 1
Show Author Affiliations
Bevin Lin, UC Davis Medical Ctr. (United States)
Dennis L. Matthews, UC Davis Medical Ctr. (United States)
Stavros G. Demos, Lawrence Livermore National Lab. (United States)
Shiro Urayama, UC Davis Health System (United States)
Ramez M. G. Saroufeem, UC Davis Health System (United States)

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