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Journal of Biomedical Optics • Open Access

Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope
Author(s): Meagan A. Saldua; Cory A. Olsovsky; Kristen C. Maitland; Evelyn S. Callaway; Robert S. Chapkin

Paper Abstract

Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at 8,333  lines/sec by a polygon scanning mirror while the specimen is scanned in the orthogonal axis by a motorized translation stage with a maximum speed of 7  mm/sec. A single 1×60 mm2 field of view image spanning the length of the mouse colon is acquired in 10 s. Z-projection images generated from axial image stacks allow high resolution imaging of the surface of non-flat specimens. In contrast to the uniform size, shape, and distribution of colon crypts in confocal images of normal colon, confocal images of chronic bowel inflammation exhibit heterogeneous tissue structure with localized severe crypt distortion.

Paper Details

Date Published: 8 February 2012
PDF: 8 pages
J. Biomed. Opt. 17(1) 016006 doi: 10.1117/1.JBO.17.1.016006
Published in: Journal of Biomedical Optics Volume 17, Issue 1
Show Author Affiliations
Meagan A. Saldua, Texas A&M Univ. (United States)
Cory A. Olsovsky, Texas A&M Univ. (United States)
Kristen C. Maitland, Texas A&M Univ. (United States)
Evelyn S. Callaway, Texas A&M Univ. (United States)
Robert S. Chapkin, Texas A&M Univ. (United States)

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