Share Email Print
cover

Journal of Biomedical Optics

Tryptophan autofluorescence imaging of neoplasms of the human colon
Author(s): Bhaskar Banerjee; Nathaniel S. Rial; Piyush Tiwari; Hemanth Gavini; Urs Utzinger; Timothy Renkoski; Logan R. Graves; Vassiliki L. Tsikitis; Valentine Nfonsom; Judith Pugh
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Detection of flat neoplasia is a major challenge in colorectal cancer screening, as missed lesions can lead to the development of an unexpected 'incident' cancer prior to the subsequent endoscopy. The use of a tryptophan-related autofluorescence has been reported to be increased in murine intestinal dysplasia. The emission spectra of cells isolated from human adenocarcinoma and normal mucosa of the colon were studied and showed markedly greater emission intensity from cancerous cells compared to cells obtained from the surrounding normal mucosa. A proto-type multispectral imaging system optimized for ultraviolet macroscopic imaging of tissue was used to obtain autofluorescence images of surgical specimens of colonic neoplasms and normal mucosa after resection. Fluorescence images did not display the expected greater emission from the tumor as compared to the normal mucosa, most probably due to increased optical absorption and scattering in the tumors. Increased fluorescence intensity in neoplasms was observed however, once fluorescence images were corrected using reflectance images. Tryptophan fluorescence alone may be useful in differentiating normal and cancerous cells, while in tissues its autofluorescence image divided by green reflectance may be useful in displaying neoplasms.

Paper Details

Date Published: 1 February 2012
PDF: 8 pages
J. Biomed. Opt. 17(1) 016003 doi: 10.1117/1.JBO.17.1.016003
Published in: Journal of Biomedical Optics Volume 17, Issue 1
Show Author Affiliations
Bhaskar Banerjee, The Univ. of Arizona College of Medicine (United States)
Nathaniel S. Rial, The Univ. of Arizona College of Medicine (United States)
Piyush Tiwari, The Univ. of Arizona College of Medicine (United States)
Hemanth Gavini, The Univ. of Arizona College of Medicine (United States)
Urs Utzinger, The Univ. of Arizona (United States)
Timothy Renkoski, College of Optical Sciences, The Univ. of Arizona (United States)
Logan R. Graves, College of Optical Sciences, The Univ. of Arizona (United States)
Vassiliki L. Tsikitis, The Univ. of Arizona College of Medicine (United States)
Valentine Nfonsom, The Univ. of Arizona College of Medicine (United States)
Judith Pugh, The Univ. of Arizona College of Medicine (United States)


© SPIE. Terms of Use
Back to Top