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Journal of Biomedical Optics • Open Access

Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy
Author(s): Deqiu Huang; Wen Zhang; Huiqing Zhong; Honglian Xiong; Xi Guo; Zhouyi Guo

Paper Abstract

In present work, we studied the effect of optical clearing on porcine skin in vitro with glycerol by Raman microspectroscopy, denoted as RM, at various time intervals of 0, 15, 30, 45, 60, and 75 min respectively. The results showed that the addition of glycerol significantly improved the depth of RM measurement, and enhanced the recovery of skin tissue Raman spectra that were not overlapped with the glycerol Raman spectra over time. Moreover, it was found that the Raman signals resembled the native spectrum of the molecules in porcine skin with a negligible frequency shift. Furthermore, we evaluated the extent of optical clearing in porcine skin by utilizing various concentrations of 40%, 60%, and 80% glycerol solution. The results demonstrated that with the increase of concentration of glycerol, the optical clearing of porcine skin was much improved.

Paper Details

Date Published: 6 February 2012
PDF: 9 pages
J. Biomed. Opt. 17(1) 015004 doi: 10.1117/1.JBO.17.1.015004
Published in: Journal of Biomedical Optics Volume 17, Issue 1
Show Author Affiliations
Deqiu Huang, South China Normal Univ. (China)
Wen Zhang, South China Normal Univ. (China)
Huiqing Zhong, South China Normal Univ. (China)
Honglian Xiong, South China Normal Univ. (China)
Xi Guo, South China Normal Univ. (China)
Zhouyi Guo, South China Normal Univ. (China)

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