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Journal of Biomedical Optics • Open Access

Investigating photoexcitation-induced mitochondrial damage by chemotherapeutic corroles using multimode optical imaging
Author(s): Jae-Youn Hwang; Daniel L. Farkas; David Lubow; Jessica Sims; Lali K. Medina-Kauwe; Harry B. Gray; Atif Mahammed; Zeev Gross

Paper Abstract

We recently reported that a targeted, brightly fluorescent gallium corrole (HerGa) is highly effective for breast tumor detection and treatment. Unlike structurally similar porphryins, HerGa exhibits tumor-targeted toxicity without the need for photoexcitation. We have now examined whether photoexcitation further modulates HerGa toxicity, using multimode optical imaging of live cells, including two-photon excited fluorescence, differential interference contrast (DIC), spectral, and lifetime imaging. Using two-photon excited fluorescence imaging, we observed that light at specific wavelengths augments the HerGa-mediated mitochondrial membrane potential disruption of breast cancer cells in situ. In addition, DIC, spectral, and fluorescence lifetime imaging enabled us to both validate cell damage by HerGa photoexcitation and investigate HerGa internalization, thus allowing optimization of light dose and timing. Our demonstration of HerGa phototoxicity opens the way for development of new methods of cancer intervention using tumor-targeted corroles.

Paper Details

Date Published: 6 February 2012
PDF: 12 pages
J. Biomed. Opt. 17(1) 015003 doi: 10.1117/1.JBO.17.1.015003
Published in: Journal of Biomedical Optics Volume 17, Issue 1
Show Author Affiliations
Jae-Youn Hwang, Cedars-Sinai Medical Ctr. (United States)
Daniel L. Farkas, SMI (United States)
David Lubow, Cedars-Sinai Medical Ctr. (United States)
Jessica Sims, Cedars-Sinai Medical Ctr. (United States)
Lali K. Medina-Kauwe, Cedars-Sinai Medical Ctr. (United States)
Harry B. Gray, California Institute of Technology (United States)
Atif Mahammed, Technion-Israel Institute of Technology (Israel)
Zeev Gross, Technion-Israel Institute of Technology (Israel)


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