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Journal of Biomedical Optics • Open Access

High-throughput optofluidic system for the laser microsurgery of oocytes
Author(s): Charlie Chandsawangbhuwana; Linda Z. Shi; Qingyuan Zhu; Michael W. Berns; Mark C. Alliegro

Paper Abstract

This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady fluid flow provided by an external computer-controlled syringe pump. This allows for easy media replacement without disturbing the oocyte location. The microfluidic and optical-laser microbeam ablation capabilities of the system were validated using surf clam (Spisula solidissima) oocytes that were immobilized in order to permit ablation of the 5 μm diameter nucleolinus within the oocyte nucleolus. Oocytes were the followed and assayed for polar body ejection.

Paper Details

Date Published: 7 February 2012
PDF: 6 pages
J. Biomed. Opt. 17(1) 015001 doi: 10.1117/1.JBO.17.1.015001
Published in: Journal of Biomedical Optics Volume 17, Issue 1
Show Author Affiliations
Charlie Chandsawangbhuwana, Univ. of California, San Diego (United States)
Linda Z. Shi, Univ. of California, San Diego (United States)
Qingyuan Zhu, Univ. of California, San Diego (United States)
Michael W. Berns, Univ. of California, San Diego (United States)
Mark C. Alliegro, Beckman Laser Institute and Medical Clinic (United States)

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