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Journal of Biomedical Optics • Open Access

Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching-based fluorescence resonance energy transfer measurements
Author(s): Arne Seitz; Stefan Terjung; Timo Zimmermann; Rainer Pepperkok

Paper Abstract

Fluorescence resonance energy transfer (FRET) efficiency measurements based on acceptor photobleaching of yellow fluorescent protein (YFP) are affected by the fact that bleaching of YFP produces a fluorescent species that is detectable in cyan fluorescent protein (CFP) image channels. The presented quantitative measurement of this conversion makes it possible to correct the obtained FRET signal to increase the accuracy of intensity based CFP/YFP FRET measurements. The described method can additionally be used to compare samples with very different fluorescence levels.

Paper Details

Date Published: 13 February 2012
PDF: 6 pages
J. Biomed. Opt. 17(1) 011010 doi: 10.1117/1.JBO.17.1.011010
Published in: Journal of Biomedical Optics Volume 17, Issue 1
Show Author Affiliations
Arne Seitz, European Molecular Biology Lab. (Germany)
Stefan Terjung, European Molecular Biology Lab. (Germany)
Timo Zimmermann, European Molecular Biology Lab. (Germany)
Rainer Pepperkok, European Molecular Biology Lab. (Germany)


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