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Optical Engineering

Chemiluminescent optical fiber immunosensor for detecting cholera antitoxin
Author(s): Robert S. Marks; Effim Bassis; Alexei Bychenko; Myron M. Levine
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Paper Abstract

A chemiluminescent-based optical fiber immunosensor is developed to detect the presence of jejunal cholera antitoxin IgA immunoglobulins. This was accomplished using optical fiber tips, conjugated with the cholera toxin B subunit. The cholera antitoxin analyte is marked by a secondary antibody labeled with horseradish peroxidase. A photoelectronic setup is designed specifically to monitor the signal. This immunosensor system is shown to be specific, sensitive, and fast to run, without requiring a purification step. The lowest titer detected was 1:1,310,720. When the luminol-containing buffer solution was replaced by air, thus dramatically lowering the index of refraction of the surrounding medium, sensitivity increased and cholera antitoxin was detected at an additional titer dilution at 1:2,621,440.

Paper Details

Date Published: 1 December 1997
PDF: 7 pages
Opt. Eng. 36(12) doi: 10.1117/1.601560
Published in: Optical Engineering Volume 36, Issue 12
Show Author Affiliations
Robert S. Marks, Ben Gurion Univ. of Negev (Israel)
Effim Bassis, Ben Gurion Univ. of the Negev (Israel)
Alexei Bychenko, Ben Gurion Univ. of the Negev (Israel)
Myron M. Levine, Univ. of Maryland School of Medicine (United States)

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