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Journal of Biomedical Optics

Study of photodynamic reactions in human blood
Author(s): Alexandre Yu. Douplik; Alexander A. Stratonnikov; Victor B. Loschenov; Victoria S. Lebedeva; Valentina M. Derkacheva; I. Alex Vitkin; V. D. Rumyanceva; S. G. Kusmin; Andrei F. Mironov; Eugeny A. Lukyanets
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Paper Abstract

Comparative studies of oxygen consumption, changes of photosensitizer fluorescence, and photodestruction of erythrocytes, and photodestruction of oxygen transport protein hemoglobin were performed during photodynamic reaction in whole and hemolyzed blood with phthalocyanines, chlorines, porphyrins, and methylene blue photosensitizers in vitro and in selected cases in vivo. The present work deals with the investigation of blood oxygen saturation SO2 and photosensitizer fluorescence during and immediately after light irradiation in the photodynamic therapy process. It has been observed that SO2 behavior strongly correlates with the type of photosensitizer. The decrease of photosensitizer fluorescence (photobleaching) during light irradiation can be followed by the recovery of the photosensitizer fluorescence immediately after interruption of the irradiation within 6–8 min. The levels of photodestruction of erythrocytes in whole blood and photodestruction of hemoglobin in hemolyzed blood in combination with the above photosensitizers reveal the influence of photodynamic reactions upon the ability of blood to transport oxygen. Maximal photohemolysis activity has been found with chlorine p6 photosensitizers.

Paper Details

Date Published: 1 July 2000
PDF: 12 pages
J. Biomed. Opt. 5(3) doi: 10.1117/1.430005
Published in: Journal of Biomedical Optics Volume 5, Issue 3
Show Author Affiliations
Alexandre Yu. Douplik, Russian Academy of Sciences (Canada)
Alexander A. Stratonnikov, General Physics Inst. (Russia)
Victor B. Loschenov, General Physics Institute (Russia)
Victoria S. Lebedeva, Institute of Fine Chemical Technology (Russia)
Valentina M. Derkacheva, NIOPIK Institute of Oraganic Intermediates & Dyes (Russia)
I. Alex Vitkin, Univ. of Toronto and Ontario Cancer Institute (Canada)
V. D. Rumyanceva, Institute of Fine Chemical Technology (Russia)
S. G. Kusmin, NIOPIK Institute of Organic Intermediates & Dyes (Russia)
Andrei F. Mironov, Lomonosov Moscow State Univ. (Russia)
Eugeny A. Lukyanets, Organic Intermediates and Dyes Institute (Russia)


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