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Journal of Biomedical Optics

Feasibility of field-based light scattering spectroscopy
Author(s): Changhuei Yang; Lev T. Perelman; Adam Wax; Ramachandra R. Dasari; Michael S. Feld
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Paper Abstract

Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible. A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 µm in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed.

Paper Details

Date Published: 1 April 2000
PDF: 6 pages
J. Biomed. Opt. 5(2) doi: 10.1117/1.429980
Published in: Journal of Biomedical Optics Volume 5, Issue 2
Show Author Affiliations
Changhuei Yang, MIT (United States)
Lev T. Perelman, Massachusetts Institute of Technology (United States)
Adam Wax, MIT (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)

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