Share Email Print

Journal of Biomedical Optics

Blood plasma coagulation studied by surface plasmon resonance
Author(s): Trine P. Vikinge; Kenny M. Hansson; Johan Benesch; Knut Johansen; Mats Ranby; Tomas L. Lindahl; Bo Liedberg; Ingemar Lundstoem; Pentti Tengvall
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

A surface plasmon resonance (SPR) apparatus was used to investigate blood plasma coagulation in real time as a function of thromboplastin and heparin concentrations. The response curves were analyzed by curve fitting to a sigmoid curve equation, followed by extraction of the time constant. Clotting activation by thromboplastin resulted in increased time constant, as compared to spontaneously clotted plasma, in a dose dependent way. Addition of heparin to the thromboplastin-activated plasma counteracted this effect. Atomic force microscopy (AFM) pictures of sensor surfaces dried after completed clotting, revealed differences in fibrin network structures as a function of thromboplastin concentration, and the fiber thickness increased with decreased thromboplastin concentration. The physical reason for the SPR signal observed is ambiguous and is therefore discussed. However, the results summarized in the plots and the fibrin network properties observed by AFM correlate well with present common methods used to analyze blood coagulation.

Paper Details

Date Published: 1 January 2000
PDF: 5 pages
J. Biomed. Opt. 5(1) doi: 10.1117/1.429968
Published in: Journal of Biomedical Optics Volume 5, Issue 1
Show Author Affiliations
Trine P. Vikinge, Linkoeping Univ. (Sweden)
Kenny M. Hansson, Linkoeping Univ. (Sweden)
Johan Benesch, Linkoeping Univ. (Sweden)
Knut Johansen, Linkoeping Univ. (Sweden)
Mats Ranby, Univ. Hospital (Sweden)
Tomas L. Lindahl, Univ. Hospital (Sweden)
Bo Liedberg, Linkoping Univ. (Sweden)
Ingemar Lundstoem, Linkoeping University (Sweden)
Pentti Tengvall, Linkoeping Univ. (Sweden)

© SPIE. Terms of Use
Back to Top