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Journal of Biomedical Optics

Polarization-based sensing of glucose using an oriented reference film
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Paper Abstract

We describe a new approach to glucose sensing using polarization measurements in the presence of a stretchoriented reference film. The method relies on measurement of the polarized emission from the reference film and of a fluorophore which changes intensity in response to glucose. A glucose-sensitive fluorescent signal was provided by the glucose/galactose binding protein from E. coli. This protein was labeled with an environmentally sensitive fluorophore at a single genetically inserted cysteine residue, and displayed decreased fluorescence upon glucose binding. Using the protein and the reference film we observed glucose-sensitive polarization values for micromolar glucose concentrations. This method of polarization-based sensing is generic and can be used for any sensing fluorophore which displays a change in intensity. In principle, one can construct simple and economical devices for this type of glucose measurement.

Paper Details

Date Published: 1 October 1999
PDF: 7 pages
J. Biomed. Opt. 4(4) doi: 10.1117/1.429955
Published in: Journal of Biomedical Optics Volume 4, Issue 4
Show Author Affiliations
Joseph R. Lakowicz, Univ. of Maryland (United States)
Ignacy Gryczynski, Univ. of Maryland School of Medicine/Baltimore (United States)
Zygmunt Gryczynski, Univ. of Maryland School of Medicine/Baltimore (United States)
Leah Tolosa, Univ. of Maryland School of Medicine (United States)
Lisa Randers-Eichhorn, Univ. of Maryland/College Park (United States)
Govind Rao, Univ. of Maryland School of Medicine (United States)


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