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Journal of Biomedical Optics

Cell viability and DNA denaturation measurements by two-photon fluorescence excitation in CW Al:GaAs diode laser optical traps
Author(s): Zhanxiang Zhang; Gregory J. Sonek; Xunbin Wei; Chung-Ho Sun; Michael W. Berns; Bruce J. Tromberg
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Paper Abstract

Cell viability and DNA denaturation are measured through two-photon fluorescence excitation using a single diode laser beam as the trapping and exciting source simultaneously. Two-photon fluorescence emission spectra are presented for CHO cells and T lymphocytes loaded with various fluorescent probes. This single beam method is demonstrated to be a safe tool to monitor the viability of optically trapped cells, even under intense 809 nm diode laser illumination (~106 W/cm2). The dynamics of cellular necrosis is monitored by adding ethanol to the cell suspension during trapping. Thermal damage to heat-treated samples is assessed by recording shifts in the emission spectra from trapped cells loaded with the nucleic acid probe, acridine orange.

Paper Details

Date Published: 1 April 1999
PDF: 4 pages
J. Biomed. Opt. 4(2) doi: 10.1117/1.429948
Published in: Journal of Biomedical Optics Volume 4, Issue 2
Show Author Affiliations
Zhanxiang Zhang, Physical Optics Corp. (United States)
Gregory J. Sonek, Beckman Laser Institute and Medical Clinic (United States)
Xunbin Wei, Univ. of California/Irvine (United States)
Chung-Ho Sun, Univ. of California/Irvine (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Univ. of California/Irvine (United States)

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