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Journal of Biomedical Optics

Topical versus systemic 5-aminolevulinic acid administration for photodynamic therapy of the colon in B10.RBP mice
Author(s): Maciej Gil; Marek Woszczynski; Jaroslaw Regula; Alexander J. MacRobert; Eugeniusz Butruk; Stephen G. Bown
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Paper Abstract

5-aminolevulinic acid (5-ALA) is an interesting photosensitizing substance for photodynamic therapy (PDT), successfully applied topically for urological malignancy. In gastroenetrology it has proven efficacy for treatment of some GI neoplasms after systemic administration. This study was aimed at investigating the possibility of topical 5-ALA administration also for the PDT of gut cancer in a mice model. 5-ALA solution at different concentrations (5%, 1.5%, and 0.5%) was instilled in the colon of mice, which was later removed and examined by fluorescence microscopy. The results of fluorescence studies were compared with those obtained in a control group treated with 5-ALA given systemically. Satisfactory epithelial fluorescence levels and good selectivity between gut layers was obtained after intracolonic 5-ALA instillation. However, mean fluorescence intensity was higher after systemic drug application. Our results suggest that 5-ALA may probably be used topically for the PDT of some gut neoplasms.

Paper Details

Date Published: 1 July 1999
PDF: 6 pages
J. Biomed. Opt. 4(3) doi: 10.1117/1.429929
Published in: Journal of Biomedical Optics Volume 4, Issue 3
Show Author Affiliations
Maciej Gil, Medical Center of Postgraduate Education (Poland)
Marek Woszczynski, Oncology Center (Poland)
Jaroslaw Regula, Medical Center of Postgraduate Education (Poland)
Alexander J. MacRobert, Univ. College London (United Kingdom)
Eugeniusz Butruk, Medical Center of Postgraduate Education (Poland)
Stephen G. Bown, Univ. College London Medical School (United Kingdom)

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