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Journal of Biomedical Optics

Photodynamic treatment of epithelial tissue derived from patients with endometrial cancer: a contribution to the role of laminin and epidermal growth factor receptor in photodynamic therapy
Author(s): Piotr P. Ziolkowski; Krzysztof Symonowicz; Beata J. Osiecka; Jerzy Rabczynski; Jerzy Gerber
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Paper Abstract

Photodynamic therapy (PDT) was used to treat endometrial G1 cancer tissue derived from patients who had undergone a total hysterectomy and bilateral salpingo-oophorectomy. After surgical treatment the cancerous tissue was kept in a medium containing Dulbecco solution, fetal calf serum, and antibiotics. The tissue was then exposed to hematoporphyrin derivative (0.1 mg/l ) and 24 h later exposed to light (total light dose—18 J/sq cm). Necrosis depth was evaluated 24 h later using a light microscope. In order to assess the possible role of the basal membrane component laminin, as well as epidermal growth factor receptor susceptibility to PDT, immunohistochemical studies were carried out. Additionally, nucleolar organizer regions evaluation was performed. Our experiment confirmed that PDT results in the necrosis in the treated endometrial cancer, while not affecting the laminin in the cancerous tissue. In contrast, PDT strongly affects the epidermal growth factor receptor and nucleolar organizer regions in cancer cells. We suggest that laminin may contribute to the prevention of cancer dissemination in the cases where PDT has to be repeated, and that after PDT the cells become less susceptible to a mitogen, like, e.g., epidermal growth factor.

Paper Details

Date Published: 1 July 1999
PDF: 4 pages
J. Biomed. Opt. 4(3) doi: 10.1117/1.429928
Published in: Journal of Biomedical Optics Volume 4, Issue 3
Show Author Affiliations
Piotr P. Ziolkowski, Medical Academy of Wroclaw (Poland)
Krzysztof Symonowicz, Medical Univ. of Wroclaw (Poland)
Beata J. Osiecka, Medical Univ. of Wroclaw (Poland)
Jerzy Rabczynski, Medical Univ. of Wroclaw (Poland)
Jerzy Gerber, Medical Univ. of Wroclaw (Poland)

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