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Journal of Biomedical Optics

Influence of the scattering phase function approximation on the optical properties of blood determined from the integrating sphere measurements
Author(s): Anna N. Yaroslavsky; Ilya V. Yaroslavsky; Thomas Goldbach; Hans-Joachim Schwarzmaier
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Paper Abstract

We investigated the impact of the scattering phase function approximation on the optical properties of whole human blood determined from integrating sphere measurements using an inverse Monte Carlo technique. The diffuse reflectance Rd and the total transmittance Tt (?=633 nm) of whole blood samples (Hct=38%) were measured with double-integrating sphere equipment. The scattering phase functions of highly diluted blood samples (Hct=0.1%) were measured using a goniophotometer. We approximated the experimentally determined scattering phase functions with either Henyey–Greenstein (HGPF), Gegenbauer kernel (GKPF), or Mie (MPF) phase functions to preset the anisotropy factor µ for the inverse problem. We have employed HGPF, GKPF, and MPF approximations in the inverse Monte Carlo procedure to derive the absorption coefficient µa and the scattering coefficient µs . To evaluate the obtained data, we calculated the angular distributions of scattered light for optically thick samples and compared the results with goniophotometric measurements. The data presented in this study demonstrate that the employed approximation of the scattering phase function can have a substantial impact on the derived values of µs and µ, while µa and the reduced scattering coefficient µ's are much less sensitive to the exact form of the scattering phase function.

Paper Details

Date Published: 1 January 1999
PDF: 7 pages
J. Biomed. Opt. 4(1) doi: 10.1117/1.429920
Published in: Journal of Biomedical Optics Volume 4, Issue 1
Show Author Affiliations
Anna N. Yaroslavsky, Louisiana State Univ. Medical Center (United States)
Ilya V. Yaroslavsky, Louisiana State Univ. (United States)
Thomas Goldbach, Heinrich-Heine-Univ. (Germany)
Hans-Joachim Schwarzmaier, German National Research Center for Information Technology (Germany)

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