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Journal of Biomedical Optics

Spatial distribution of phosphate species in mature and newly generated mammalian bone by hyperspectral Raman imaging
Author(s): Jerilyn A. Timlin; Angela Carden; Michael D. Morris; Jeffrey F. Bonadio; C. Edward Hoffler; Kenneth Kozloff; Steven A. Goldstein
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Paper Abstract

Hyperspectral Raman images of mineral components of trabecular and cortical bone at 3 µm spatial resolution are presented. Contrast is generated from Raman spectra acquired over the 600–1400 cm?1 Raman shift range. Factor analysis on the ensemble of Raman spectra is used to generate descriptors of mineral components. In trabecular bone independent phosphate (PO4?3) and monohydrogen phosphate (HPO4?2) factors areobserved. Phosphate and monohydrogen phosphate gradients extend from trabecular packets into the interior of a rod. The gradients are sharply defined in newly regenerated bone. There, HPO4?2 content maximizes near a trabecular packet and decreases to a minimum value over as little as a 20 µm distance. Incomplete mineralization is clearly visible. In cortical bone, factor analysis yields only a single mineral factor containing both PO4?3 and HPO4?2 signatures and this implies uniform distribution of these ions in the region imaged.Uniform PO4?3 and HPO4?2 distribution is verified by spectral band integration.

Paper Details

Date Published: 1 January 1999
PDF: 7 pages
J. Biomed. Opt. 4(1) doi: 10.1117/1.429918
Published in: Journal of Biomedical Optics Volume 4, Issue 1
Show Author Affiliations
Jerilyn A. Timlin, Univ. of Michigan (United States)
Angela Carden, Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)
Jeffrey F. Bonadio, Univ. of Michigan Medical School (United States)
C. Edward Hoffler, Univ. of Michigan Medical School (United States)
Kenneth Kozloff, Univ. of Michigan Medical School (United States)
Steven A. Goldstein, Univ. of Michigan Medical School (United States)

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