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Journal of Biomedical Optics

Infrared spectroscopic imaging of the biochemical modifications induced in the cerebellum of the Niemann-Pick type C mouse
Author(s): Linda H. Kidder; Pina Colarusso; Sarah A. Stewart; Ira W. Levin; Nathan M. Appel; David S. Lester; Peter G. Pentchev; E. Neil Lewis
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Paper Abstract

We have applied Fourier transform infrared (IR) spectroscopic imaging to the investigation of the neuropathologic effects of a genetic lipid storage disease, Niemann–Pick type C (NPC). Tissue sections both from the cerebella of a strain of BALB/c mice that demonstrated morphology and pathology of the human disease and from control animals were used. These samples were analyzed by standard histopathological procedures as well as this new IR imaging approach. The IR absorbance images exhibit contrast based on biochemical variations and allow for the identification of the cellular layers within the tissue samples. Furthermore, these images provide a qualitative description of the localized biochemical differences existing between the diseased and control tissue in the absence of histological staining. Statistical analyses of the IR spectra extracted from individual cell layers of the imaging data sets provide concise quantitative descriptions of these biochemical changes. The results indicate that lipid is depleted specifically in the white matter of the NPC mouse in comparison to the control samples. Minor differences were noted for the granular layers, but no significant differences were observed in the molecular layers of the cerebellar tissue. These changes are consistent with significant demyelination within the cerebellum of the NPC mouse.

Paper Details

Date Published: 1 January 1999
PDF: 7 pages
J. Biomed. Opt. 4(1) doi: 10.1117/1.429915
Published in: Journal of Biomedical Optics Volume 4, Issue 1
Show Author Affiliations
Linda H. Kidder, National Institutes of Health (United States)
Pina Colarusso, National Institutes of Health (United States)
Sarah A. Stewart, National Institutes of Health (United States)
Ira W. Levin, National Institutes of Health (United States)
Nathan M. Appel, Food and Drug Administration (United States)
David S. Lester, FDA Div. of Research and Testing (United States)
Peter G. Pentchev, National Institutes of Health (United States)
E. Neil Lewis, National Institutes of Health (United States)

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