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Journal of Biomedical Optics

Articular cartilage optical properties in the spectral range 300--850 nm
Author(s): Daniel W. Ebert; Cynthia J. Roberts; Stuart K. Farrar; William M. Johnston; Alan S. Litsky; Alicia L. Bertone
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Paper Abstract

Measurements of absolute total reflectance were recorded from weight-bearing (n=9) and nonweight-bearing (n=9) equine articular cartilage specimens from 300 to 850 nm using a spectrophotometer with integrating sphere attachment. Following correction of measured spectra for interfacial reflections and edge losses, Kubelka–Munk theory was applied to estimate absorption and scattering coefficient, one-dimensional light intensity distribution, and light penetration depth. Kubelka–Munk absorption coefficients ranged from ~7 cm?1 at 330 nm to ~1 cm?1 at 850 nm. A localized absorption peak was noted at ~340 nm. Above 510 nm, weight-bearing cartilage demonstrated significantly higher absorption coefficients than nonweight-bearing tissue (paired t-test, p<0.05). Kubelka–Munk scattering coefficients ranged from~40 cm?1 at 360 nm to ~6 cm?1 at 850 nm. No statistical differences in scattering coefficient were noted between weight-bearing and nonweight-bearing tissue. Penetration depths predicted by Kubelka–Munk theory ranged from 0.6 mm at 350 nm to over 3 mm at 850 nm. Stronger absorption in weight-bearing cartilage compared to nonweight-bearing tissue resulted in lower light penetration depths in weight-bearing cartilage at all wavelengths longer than 510 nm.

Paper Details

Date Published: 1 July 1998
PDF: 8 pages
J. Biomed. Opt. 3(3) doi: 10.1117/1.429893
Published in: Journal of Biomedical Optics Volume 3, Issue 3
Show Author Affiliations
Daniel W. Ebert
Cynthia J. Roberts, The Ohio State Univ. (United States)
Stuart K. Farrar, Ohio State Univ. (United States)
William M. Johnston, Ohio State Univ. (United States)
Alan S. Litsky, Ohio State Univ. (United States)
Alicia L. Bertone, Ohio State Univ. (United States)

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