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Journal of Biomedical Optics • Open Access

Effect of cross-linking with riboflavin and ultraviolet A on the chemical bonds and ultrastructure of human sclera
Author(s): Gyeong-Bok Jung; Ji-Hye Kim; Jin Ik Lim; Samjin Choi; Hun-Kuk Park; Hui-Jae Lee; Kyung-Hyun Jin

Paper Abstract

This study examined the effect of the cross-linking with riboflavin-ultraviolet A (UVA) irradiation on the chemical bonds and ultrastructural changes of human sclera tissues using Raman spectroscopy and atomic force microscopy (AFM). Raman spectroscopy of the normal and cross-linked human sclera tissue revealed different types of the riboflavin-UVA and collagen interactions, which could be identified from their unique peaks, intensity, and shape. Raman spectroscopy can prove to be a powerful tool for examining the chemical bond of collagenous tissues at the molecular level. After riboflavin-UVA treatment, unlike a regular parallel arrangement of normal collagen fibrils, the AFM image revealed interlocking arrangements of collagen fibrils. The observed changes in the surface topography of the collagen fibrils, as well as in their chemical bonds in the sclera tissue, support the formation of interfibrilar cross-links in sclera tissues.

Paper Details

Date Published: 1 December 2011
PDF: 7 pages
J. Biomed. Opt. 16(12) 125004 doi: 10.1117/1.3662458
Published in: Journal of Biomedical Optics Volume 16, Issue 12
Show Author Affiliations
Gyeong-Bok Jung, Kyung Hee Univ. (Korea, Republic of)
Ji-Hye Kim, Kyung Hee Univ. (Korea, Republic of)
Jin Ik Lim, Kyung Hee Univ. (Korea, Republic of)
Samjin Choi, Kyung Hee Univ. (Korea, Republic of)
Hun-Kuk Park, Kyung Hee Univ. (Korea, Republic of)
Hui-Jae Lee, Kangwon National Univ. (Korea, Republic of)
Kyung-Hyun Jin, Kyung Hee Univ. (Korea, Republic of)

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