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Journal of Biomedical Optics • Open Access

Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy
Author(s): Honghai Liu; Wan Qin; Zhen Ma; Bruce Z. Gao; Yonghong Shao; Tong Ye; Thomas K. Borg

Paper Abstract

We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.

Paper Details

Date Published: 1 December 2011
PDF: 5 pages
J. Biomed. Opt. 16(12) 126012 doi: 10.1117/1.3662457
Published in: Journal of Biomedical Optics Volume 16, Issue 12
Show Author Affiliations
Honghai Liu, Clemson Univ. (United States)
Wan Qin, Clemson Univ. (United States)
Zhen Ma, Clemson Univ. (United States)
Bruce Z. Gao, Clemson Univ. (United States)
Yonghong Shao, Shenzhen Univ. (China)
Tong Ye, The Univ. of Alabama at Birmingham (United States)
Thomas K. Borg, Medical Univ. of South Carolina (United States)


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